Diet Research Data：Effects of Quick Fat Diet on Male C57BL/6J Mice

 

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１.Objective

This study examined the effects of a high-fat diet (Quick Fat; QF) on male C57BL/6J mice.

 

２.Materials and Methods

（1）Experimental Site

The experiment was conducted in the mouse breeding room (conventional) of the Central Research Institute of Nippon Compound Feed Co., Ltd. (currently Feed One Co., Ltd.).

（2）Diet

• CE-2: Crude fat content 4.6%, Fat kcal% 11.9%, Soluble non-nitrogenous substance content 51.4%, NFE kcal% 59.3%
• Quick Fat (QF): Crude fat content 13.6%, Fat kcal% 29.8%, Soluble non-nitrogenous substance content 47.8%, NFE kcal% 46.6%

（3）Animals

Male C57BL/6JJcl mice were used as subjects (20 mice per group, 40 mice in total).

（4）Housing

• Temperature and humidity: Temperature = 21-25°C, Humidity = 40-60%
• Lighting: 12-hour light-dark cycle (lights on 9:00-21:00)
• Cages: Individually housed in polycarbonate cages with sterilized wood chips
• Diet: Ad libitum
• Drinking water: Ad libitum

（5）Experimental Procedure

C57BL/6J mice were introduced at 4 weeks of age and acclimatized for 1 week (fed CE-2). The mice were then divided into two groups (CE-2 group and QF group) based on body weight and blood glucose levels to ensure no differences between groups. The experimental diet was fed from 5 weeks of age to 20 weeks of age (feeding period: 16 weeks), and various measurements were performed.

 

３.Results

The results are described below.

 

1.Body Weight

Body Weight Changes

Body weight was measured weekly. The points and vertical lines in the figure represent the mean ± standard error. Statistical analysis was performed between groups at each age, and * indicates a significant difference (p < 0.05).

 

2.Food and Water Intake

Food Intake

The points and vertical lines in the figure represent the mean ± standard error. Statistical analysis was performed between groups at each age, and * indicates a significant difference (p < 0.05). Food intake represents the average daily intake per mouse at each age.

Water Intake

Water intake represents the average daily intake per mouse at each age. The points and vertical lines in the figure represent the mean ± standard error. Statistical analysis was performed between groups at each age, and * indicates a significant difference (p < 0.05).

 

3.Fasting Blood Glucose Levels

Changes in Fasting Blood Glucose Levels

Blood samples were collected from the tail vein every 3 weeks at 1 pm, 4 hours after lights on. Whole blood was measured using a whole blood glucose meter (Glucotest PROR / Sanwa Kagaku Kenkyusho Co., Ltd.). The vertical bars and lines in the figure represent the mean ± standard error. Statistical analysis was performed between groups at each age, and * indicates a significant difference (p < 0.05).

 

4.Oral Glucose Tolerance Test

Results of Oral Glucose Tolerance Test at Each Age - 1

The points and vertical lines in the figure represent the mean ± standard error. Statistical analysis was performed between groups at each time point, and * indicates a significant difference (p < 0.05). The test was conducted at 5, 15, and 20 weeks of age (2-3 days, 10 weeks, and 15 weeks after starting the experimental diet; shown in the upper left, upper right, and lower left panels, respectively). After an overnight fast (20 hours), a glucose solution (2 g/kg body weight) was orally administered via gastric gavage, and blood was collected from the tail vein before administration (0 min) and 30, 60, and 120 min after administration. Whole blood was measured using a whole blood glucose meter (Glucotest PROR / Sanwa Kagaku Kenkyusho Co., Ltd.).

Results of Oral Glucose Tolerance Test at Each Age – 2

【sum of blood glucose levels】

The sum of blood glucose levels was calculated for each time point during the oral glucose tolerance test. The vertical bars and lines in the figure represent the mean ± standard error. Statistical analysis was performed between groups at each age, and * indicates a significant difference (p < 0.05). Note that the results at 5 weeks of age represent those 3 days after starting the experimental diet.

【blood glucose increment area】

The vertical bars and lines in the figure represent the mean ± standard error. Statistical analysis was performed between groups at each age, and * indicates a significant difference (p < 0.05). The blood glucose increment area was calculated using the following formula:

Blood glucose increment area (mg/dl・h) = {(a + 2b + 3c + 2d) × 1/4} - 2a
(where a, b, c, and d are the blood glucose levels at 0, 30, 60, and 120 minutes, respectively).

Note that the results at 5 weeks of age represent those 3 days after starting the experimental diet.

 

5.Insulin Tolerance Test

Changes in Results of Insulin Tolerance Test at Each Age

Blood samples were collected from the tail vein every 3 weeks at 1 pm, 4 hours after lights on. Whole blood was measured using a whole blood glucose meter (Glucotest PROR / Sanwa Kagaku Kenkyusho Co., Ltd.). The vertical bars and lines in the figure represent the mean ± standard error. Statistical analysis was performed between groups at each age, and * indicates a significant difference (p < 0.05).

 

6.Blood Analysis at Biopsy

Blood lipid-related substances and other component concentrations at Biopsy (20 weeks of age)

 

7.Major Organ Weights at Biopsy

The values in the table represent the mean ± standard error. Animals were sacrificed at 20 weeks of age (n = 8 per group). After a 20-hour fast, the abdomen was opened under ether anesthesia, and each organ was removed and weighed. The upper table shows the absolute weights of each organ, and the lower table shows the relative weights per 100 g body weight. Statistical analysis was performed between groups for each item, and * indicates a significant difference (p < 0.05).

Major organ and regional adipose tissue weights at Biopsy (20 weeks of age)

Relative major organ and regional relative adipose tissue weights at Biopsy (20 weeks of age)