High-Fat Diet-Induced Obesity (DIO) Model:Histopathological Changes in Liver and Kidney after HFD32 and QF Feeding

Histopathological Analysis Results

Animal/Age	C57BL/6J mice / 24 weeks old
Necropsy Location	Ishibe Breeding Farm
Necropsy Date	2024/12/10-13
Sex	♂♀
Dietary Conditions	CE-2, HFD32, QF
n Number	5 animals per group / Total 30 (males and females combined)
Organs for Histopathological Analysis	Liver, Kidney (Evaluation by scoring after H&E staining)
Statistical Analysis	Difference between diets: Kruskal-Wallis / Sex difference: Mann–Whitney

Both HFD32 and QF induced hepatic steatosis (fatty change of hepatocytes), but it was more pronounced in HFD32 for both males and females.
Deposited lipid droplets were of larger size in females.
Inflammatory cell infiltration was induced to a very mild degree in males.

Vacuolar degeneration of tubular epithelial cells was observed in males with both HFD32 and QF.
Inflammation in the renal pelvis region was observed more frequently in the female HFD32 group.
Glomerular lesions tended to increase slightly with both HFD32 and QF.