*C57BL/6J mice cannot be shipped outside of Japan due to licensing agreements. Please consider using our contract research services if you need to use this strain.
Unlock new research pathways with reliable foundational data. We understand the critical need for well-documented animal models in aging research. This study provides a comprehensive overview of key physiological parameters in aged mice, offering valuable insights that can inform your experimental design. Accessing these transparent, verifiable datasets is the first step toward building more robust and reproducible studies.
Study Overview
This study was conducted to collect and publish basic physiological data from aged C57BL/6JJcl (B6J) and C57BL/6NJcl (B6N) mice during their geriatric phase (62-78 or 64-78 weeks of age), intended as a reference for aging research.By providing essential background information for research planning and model animal selection, this data contributes to advancing research on age-related diseases.
Materials and Methods
Animals• C57BL/6JJcl (B6J): Started at 62 weeks of age
• C57BL/6NJcl (B6N): Started at 64 weeks of age
• Each group consisted of 30 male mice, maintained until 78 weeks of age.
Housing Conditions
• Diet: CE-2 (Autoclaved), ad libitum
• Temperature: 20-26°C, Humidity: 45-70%
• Lighting: 12-hour light / 12-hour dark cycle
• Single-caged (M cage)
• Sterilized water, ad libitum
Measurement Methods
• Body Weight: Once every two weeks
• At 78 weeks, organ collection (liver, pancreas, kidney, heart, lungs, soleus muscle, eyes, brain, femur) were performed.
• Organ weights were measured, and samples were fixed in formalin for histopathological analysis (partially transferred to Study No. 2).
• Blood was collected from the posterior vena cava for blood biochemistry tests (Glucose, TG, T-cho, ALT, AST, BUN, CRE, UP, Insulin).
• Statistical Analysis: Student’s t-test (p < 0.05, p < 0.01)
Results Summary
Time-Course Changes in Body Weight
Organ Weights
• C57BL/6N strain
• C57BL/6J strain
• System-specific characteristics of each organ weight were recorded.
• Muscle weight (soleus) was also measured.
Key Biopsy Findings
Blood Biochemical Parameters
Summary
• The C57BL/6N mice showed significantly higher body weight compared to C57BL/6J mice throughout all weeks.• Mesenteric lymph node hypertrophy was the most frequently observed finding, with a clear strain difference: 20% in C57BL/6N and 3.3% in C57BL/6J.
• C57BL/6N mice had significantly higher insulin levels than C57BL/6J mice. In contrast, C57BL/6J mice showed significantly higher BUN levels and significantly lower Cre levels than C57BL/6N mice. This study confirmed significant differences between the two strains.
Related Links
• [Study: Liver and Kidney Histopathology Evaluation Data](TBD)
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