Aging Mouse Basic Data Collection Study on C57BL/6JJcl and C57BL/6NJcl Mice at 78 Weeks of Age

Study Overview

This study was conducted to collect and publish basic physiological data from aged C57BL/6JJcl (B6J) and C57BL/6NJcl (B6N) mice during their geriatric phase (62-78 or 64-78 weeks of age), intended as a reference for aging research.

By providing essential background information for research planning and model animal selection, this data contributes to advancing research on age-related diseases.

Materials and Methods

Animals

• C57BL/6JJcl (B6J): Started at 62 weeks of age
• C57BL/6NJcl (B6N): Started at 64 weeks of age
• Each group consisted of 30 male mice, maintained until 78 weeks of age.

Housing Conditions

• Diet: CE-2 (Autoclaved), ad libitum
• Temperature: 20-26°C, Humidity: 45-70%
• Lighting: 12-hour light / 12-hour dark cycle
• Single-caged (M cage)
• Sterilized water, ad libitum

Measurement Methods

• Body Weight: Once every two weeks
• At 78 weeks, organ collection (liver, pancreas, kidney, heart, lungs, soleus muscle, eyes, brain, femur) were performed.
• Organ weights were measured, and samples were fixed in formalin for histopathological analysis (partially transferred to Study No. 2).
• Blood was collected from the posterior vena cava for blood biochemistry tests (Glucose, TG, T-cho, ALT, AST, BUN, CRE, UP, Insulin).
• Statistical Analysis: Student’s t-test (p < 0.05, p < 0.01)

Results Summary

Time-Course Changes in Body Weight

Organ Weights

C57BL/6N strain

C57BL/6J strain

• System-specific characteristics of each organ weight were recorded.
• Muscle weight (soleus) was also measured.

Key Biopsy Findings

Blood Biochemical Parameters

Summary

• The C57BL/6N mice showed significantly higher body weight compared to C57BL/6J mice throughout all weeks.
• Mesenteric lymph node hypertrophy was the most frequently observed finding, with a clear strain difference: 20% in C57BL/6N and 3.3% in C57BL/6J.
• C57BL/6N mice had significantly higher insulin levels than C57BL/6J mice. In contrast, C57BL/6J mice showed significantly higher BUN levels and significantly lower Cre levels than C57BL/6N mice. This study confirmed significant differences between the two strains.

Related Links

• [Study: Liver and Kidney Histopathology Evaluation Data]

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