Examples of Previous Service: Brain and Plasma Collection Services

 

Related CLEA Japan product: BALB/cAJcl-nu/nu

For the animal, please click here↓
: https://www.clea-japan.com/en/products/immunodeficiency/item_a0010

 

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Sample Collection: Mouse BALB/cAJcl-nu/nu, 8 weeks old, male
20 ml pooled plasma, brains from 10 mice Procedure

 

①Animal Preparation

Prepare approximately 70 BALB/c nude mice.

 

②Plasma Collection

Anesthetize the animals by inhaling isoflurane and collect blood from the abdominal aorta under anesthesia.
Add heparin sodium to the collected blood, centrifuge at 2000G for 15 minutes at 4°C, and collect the plasma.

 

③Organ Collection

After euthanizing the animals from which blood was collected, collect the brains.

 

④Shipping

Freeze the collected brains and plasma at -30°C and ship them to the customer.

 

Interview of Research Administrator

 

・What kind of customers inquire about this service?

We often receive requests from CROs. I think they probably want to obtain control data.
We also receive similar inquiries from pharmaceutical companies, but I think they probably want to know in advance the components of the blood of our BALB/c nude mice, the amounts of specific proteins and vitamins, the number of cells, and etc.
I think they make the final decision on whether or not to use after confirming these data.

 

・How many similar inquiries do you receive per year?

It is a rare case to have an order for both the brain and plasma together once a year. If other organs are also extracted, it is done with a certain frequency. Including those cases, I have the impression that brain extraction is performed about 2-3 times a year.

 

・What are the precautions taken when conducting this work?

Brain collection requires very careful work with tweezers. A certain amount of experience and familiarity may be necessary.

 

・What are the advantages of requesting this service from CLEA Japan?

First of all, I think it would be very difficult to prepare the equipment. At CLEA Japan, we have installed a large dissection table equipped with a ventilation system and have taken various safety measures such as an anesthetic apparatus. It would not be easy for an institution that only works occasionally to prepare the same equipment as ours.

As a precaution for blood collection, we adopt blood collection from the abdominal aorta as quickly as possible (so that the collected blood does not coagulate) to collect clean plasma. This requires training and experience. At our company, it takes at least 3 months of training to acquire the skills.

Furthermore, under anesthesia, blood vessels may become narrower, and depending on the system, the location and condition of the arteries may vary slightly. In obese animals, blood vessels may be completely covered with fat and very difficult to see. If the artery is punctured, it can lead to massive bleeding, so skilled techniques and experience are required.

Considering the arrangement of animals and etc., it would be highly advantageous to request this service from our company, which can collect samples frequently and arrange for animals from adjacent facilities.

 

・What options are available?

Occasionally, we receive requests to measure the weight of the organs before delivery. We can also set the freezing temperature. Although the basic storage temperature is -80°C, we can also accommodate requests to send it at -20°C.

We can also accommodate requests according to the conditions of the reference paper, such as centrifugation conditions and anticoagulant designation. Regarding the containers to be stored, even if it is the same 10 mL, we can accept options such as storing in one container or storing 1 mL in 10 containers.

 

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