Please consider using High Fat Diet 32 (HFD32) for your obesity research or other dietary induction studies.

Corresponding of complains about general high-fat diets for mice and rats is too low, CLEA Japan developed HFD32 in increasing fat from 20% to 32% with solid shape. Please consider using CLEA Japan’s HFD 32 for obesity research and other dietary induction studies.

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History of development

Previously, high-fat diets for mice and rats were difficult to solidify when the amount of added fat exceeded 20% and were adjusted as powdered shape. In addition, even if a solid high-fat feed with an oil addition of 20% or less was available, it had to be imported from overseas manufacturers or adjusted by users.

Therefore, CLEA Japan undertook the task of solidifying high-fat feed, which had been difficult at that time. They succeeded in commercializing "High Fat Diet 32," a solid high-fat feed with a crude fat content of approximately 32%, which was the first domestic manufacturer to do it.

Characteristic

HFD32 is a super-high-fat diet with a crude fat content of 32% and a fat-derived calorie ratio of about 60% of total energy.

Previously, high-fat diets with the same level of fat content were only available in powdered shape. However, HFD32 is available in a more convenient pellet shape.

Since the accumulation of visceral fat is significantly observed, it is also effective for obesity research in addition to diabetes research.

Shape / Capacity

Notes:

Keep in a freezer

HFD32 is a very fragile diet at room temperature and does not maintain sufficient hardness. It is also prone to lipid oxidation.

HFD32 is delivered frozen at -20℃ immediately after manufacturing and transported in a frozen state. It should be kept in a freezer.

Feeding animals, the diet should be returned to room temperature for 1 hour when stored at -20℃ and 3 hours when stored at -80℃.

Diet replacement schedule

Please replace the HFD32 that has been fed within 2 days.

Reference data

• Male mice of the C57BL/6JJcl and KK-Ay/TaJcl strains at 4 weeks of age are divided into two groups for each strain (HFD32 and CE-2 [normal diet]).
• Free-choice diet is continued from 5 to 12 weeks of age.
• Weight is measured weekly. Oral glucose tolerance test (OGTT) and dissection are performed at 12 weeks of age.
• In the OGTT, glucose solution (2 g/kg body weight) is administered orally after 20 hours of fasting. Blood is collected from the tail vein at 0, 30, 60, and 120 minutes after administration to measure blood glucose levels.

Body weight change

Shows a significant weight gain.

Oral glucose tolerance test results

Promotes impaired glucose tolerance.

Intra-abdominal fat weight

There is a significant increase in visceral fat.

Ingredients of HFD32

General composition values (per 100g of diet)

*1  ：Calories are calculated by multiplying crude protein: 4, crude fat: 9, and soluble nitrogen-free substances (NFE): 4.

Fatty acid content (per 100g of diet)

Other HFD32 test data

The effects of different concentrations of sucrose solution on glucose tolerance in high-fat diet-fed mice

*Photo of liver tissue for each test group

In addition to feeding HFD32, we observed changes in mice after providing them with different methods of drinking sugar and fructose solutions.

▶ The effects of different concentrations of sucrose solution on glucose tolerance in high-fat diet-fed mice（PDF）

The effects of different light-dark cycles on glucose tolerance in high-fat diet-fed mice

*Incremental Area Under the Curve (IAUC)


We investigated the comparison of metabolic syndrome between morning and evening types in mice. By changing the light-dark cycle suitable for mice while feeding HFD32, we investigated the modulation of circadian rhythms.

▶ The effects of different light-dark cycles on glucose tolerance in high-fat diet-fed mice（PDF）

The effects of the presence or absence of radiation sterilization on the preservation of high-fat feed

*The changes in acidity (AV) and peroxide value (POV)

We analyzed how the different nutrients in HFD32 are affected by irradiation and how this affects the difference in preservation.

▶ The effects of the presence or absence of radiation sterilization on the preservation of high-fat feed（PDF）

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