SDT fatty Rat – Multisystem T2D Complications Model

This page summarizes SDT fatty data highlighted for ATTD-ASIA 2025.

 

Kidney ・ Retina ・ Heart ・ AI Pathology ・ Nerve

 

• Diabetic Kidney Disease (Hyperfiltration → GFR decline → fibrosis)
• Early Diabetic Retinopathy (ERG deficits · GFAP gliosis)
• HFpEF-like cardiomyopathy (E/A ratio · LVEDP)
• Fully compatible with AI-based pathology

 

Key Organ Phenotypes:

SDT fatty rats develop multi-organ complications of type 2 diabetes, allowing integrated evaluation of kidney, retina, heart, nerve and histopathological endpoints.

 

Kidney – DKD

Source: Poster CKD Summit 2024 – FITC-sinistrin GFR curve + FibroNest fibrosis map

Sample Image

Dapagliflozin strongly reduces hyperglycemia and prevents GFR decline in Unx SDT fatty rats

Fed glycemia (upper panel) and glomerular filtration rate (lower panel) in SD rats, sham or Unx SDT fatty rats on a 0.3% salt diet without or with dapagliflozin for 10 weeks.
*p<0.05, ** p<0.01, *** p<0.001 and **** p<0.0001

 

FibroNest platform uncovers the antifibrotic effects of dapagliflozin in the renal cortex of Unx SDT fatty rats

Representative pictures showing the panels illustrating original Sirius Red images and image analysis layers (upper panel), and phenotypic fibrosis composite scores (lower panel) assessed with PharmaNest's high resolution quantitative digital pathology in SD rats, sham or Unx SDT fatty rats.
*p<0.05, ** p<0.01, *** p<0.001 and **** p<0.0001

 

• Hyperfiltration → >50% GFR decline
• Glomerulosclerosis · tubular injury · fibrosis
• Responds to SGLT2i, GLP-1RA, ACEi

 

Poster CKD Summit 2024.pdf

 

Retina – Early DR

Source: ARVO 2018 – ERG deficits & GFAP gliosis

Sample Image

Electroretinography demonstrates retinal neurologic dysfunction in Unx SDT fatty rats from 12 weeks of age

A-wave (panel A; photoreceptor response) and B-wave (panel B; inner retinal cells activity) during a standard scotopic ERG recording to a bright white flash (0 log cd s/m2). Amplitudes (panel C) and implicit times (panel D) of the oscillatory potentials (OPs, reflects inner retinal function) extracted from scotopic responses.
*p<0.05 and *** p<0.001 vs. Unx SD control.

 

Unx SDT fatty rats show reactive gliosis in retina at 17 weeks of age

DAPI (4',6-diamidino-2-phenylindole; for nuclear acid staining), glial fibrillary acidic protein (GFAP) and vimentin (markers of Müller cells) immunoreactivity in sagittal retinal sections, assessed by fluorescence microscopy.
Upper and lower panels show representative pictures from a Unx SD rat and Unx SDT fatty rats, respectively, at 17 weeks of age.

 

• ERG deficits (A/B/OPs)
• GFAP/Vimentin gliosis
• Vascular dilation & remodeling

 

Poster ARVO 2018- Briand et al. (draft-1).pdf

 

Heart – HFpEF-like

Source: Mini-oral HFpEF data – E/A decline & LVEDP elevation

Sample Image

 

• Preserved EF · diastolic dysfunction
• LVEDP elevation
• Responds to GLP-1 agonists

 

220517 Briand et al. mini-oral presentation - SDT fatty rat.pdf

 

AI-based Pathology

Source: ASN 2024 – NephroPath · PEMP · FibroNest overlays

Sample Image

Nephropath Al demonstrates that dapagliflozin reduces tubuli impairment and glomerulosclerosis in Unx SDT fatty rats

Representative raw PAS staining and Nephropath Al multi-class prediction images (upperpanel), number of atrophic and dilated tubuli and sclerotic glomeruli (lower panel) in SD rats,sham or Unx SDT fatty rats on a 0.3% salt diet without or with dapagliflozin for 10 weeks.
*p<0.05, ** p<0.01, *** p<0.001 and **** p<0.0001

 

PEMP reveals that dapagliflozin improves podocyte effacement in Unx SDT fatty rats

Representative images of double staining for podocin/integrin a3 and maximum intensity projection with color coding for depth (left panel); Filtration Slit Length (FSL) and Filtration Slit Density (FSD) determined by PEMP (right panel) in SD rats, sham or Unx SDT fatty rats.
*p<0.05, ** p<0.01 and **** p<0.0001

 

FibroNest platform uncovers the antifibrotic effects of dapagliflozin in the renal cortex of Unx SDT fatty rats

Representative pictures showing the panels illustrating original Sirius Red images and image analysis layers (upper panel), and phenotypic fibrosis composite scores (lower panel) assessed with PharmaNest's high resolution quantitative digital pathology in SD rats, sham or Unx SDT fatty rats.
*p<0.05, ** p<0.01, *** p<0.001 and **** p<0.0001

 

• NephroPath – glomerular analysis
• PEMP – podocyte effacement
• FibroNest – fibrosis depth/volume map

 

Physiogenex - poster ASN Kidney Week 2024 - SDT fatty rat.pdf

 

Nerve – Peripheral Neuropathy

Source: ATTD-ASIA 2025 Poster – NF-L reduction by dapagliflozin

Sample Image (NF-L: plasma neurofilament light chain)

 

• NF-L elevation indicates peripheral nerve injury in the Unx SDT fatty rat
• Dapagliflozin lowers NF-L, suggesting therapeutic improvement
• Neuropathy appears as an additional comorbidity alongside nephropathy and retinopathy

 

ATTD-ASIA 2025 e-Poster(TBD)

 

Key Organ Phenotypes:

• Physiogenex (France)– DKD, HFpEF, and metabolic complications

 

• Cardiomedex (France)– Cardiac imaging, HFpEF characterization, and cardiometabolic phenotyping」

• AI pathology partners – Aiosyn, Nipoka, PharmaNest, etc.

These partners play a central role in generating and validating SDT fatty data for global pharmaceutical R&D.

 

Applications

• DKD progression & renoprotective drug evaluation
• HFpEF / cardiorenal axis studies
• Early DR neurovascular research
• AI pathology validation & digital biomarker development

 

ATTD-ASIA 2025 e-Poster

SGLT2 INHIBITION IMPROVES GLYCEMIC CONTROL, DIABETIC NEPHROPATHY AND NEUROPATHY IN THE SPONTANEOUSLY DIABETIC TORII FATTY RAT, A TRANSLATIONAL MODEL OF TYPE 2 DIABETES

François Briand¹, Estelle Grasset¹, Takeshi Ohta², Masami Shinohara³, Caroline Dubroca⁴, Thierry Sulpice<sup>1,4

1</sup>Physiogenex, FRANCE
²Graduate School of Agriculture, Kyoto University, JAPAN
³CLEA Japan Inc, JAPAN
⁴Cardiomedex, FRANCE

 

This work will be presented as an e-Poster at ATTD-ASIA 2025.
Please search for “SDT fatty” or “SGLT2 inhibition” in the e-Poster system. 

 

Contact

For collaboration, joint studies, or model access, please contact:
Confidential discussion available upon request.

 

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