Diet Research Data：Quick Fat Storage Test

 

Related CLEA Japan product: Quick Fat

For the animal, please click here↓
: https://www.clea-japan.com/en/products/general_diet/item_d0070

 

Inquiry:

If you have any question, please feel free to contact us from here.

 

１.Storage Test Results of Sealed Quick Fat Packages (6 months)

A storage test was conducted on Quick Fat, which has a higher fat content than regular feed, under room temperature conditions.

Feed: Quick Fat manufactured on June 13, 2003.

Packaging: Subdivided from 10 kg bags and sealed in a laminated single layer with a desiccant.

Storage temperature: Room temperature (20-25°C)

Analysis items: Acid value (AV), peroxide value (POV), retinol (VA efficacy calculated), vitamin E, B1, B12

Analysis dates: Mid-June to mid-December 2003, at the beginning of storage (mid-June), after 3 months (mid-September), and after 6 months (mid-December)

Table 1: Storage Test Results of Sealed Quick Fat Packages

Values in parentheses indicate relative values when 0 months (start) is taken as 100%.
*Calculated from retinol amount

• Under the conditions of this test, there were no significant changes in vitamins even after 6 months of storage.

• However, the acid value (AV) showed higher values as the storage period increased. Moreover, the peroxide value (POV) showed a high value after 3 months and then a low value after 6 months.

• When sealed Quick Fat is stored at room temperature within a range of 20-25°C, changes in vitamins are very small, but some progress in oil and fat changes was considered.

• It is considered that this change in oil and fat is lower than the level that affects the fed animals and does not affect the experiment.

• As shown in Table 2, it was observed that the initial values of AV and POV immediately after manufacturing varied greatly between lots. In the future, we intend to collect more analytical data to understand the degree of variation between lots.

Table 2: Reference data: Measurement results of AV and POV in different lots of Quick Fat

 

２.Changes in Quick Fat after Opening (2 weeks)

（1）Objective

The purpose of this study was to observe changes in the fat in the feed when each feed was placed in a mouse feeder.

（2）Materials and Methods

Feed that was opened and left standing in a laboratory with controlled temperature and humidity was sampled at regular intervals, and the acid value (AV) and peroxide value (POV) were analyzed to observe changes in the fat in the feed.

① Test Feed：

Quick Fat, manufactured on March 13, 2004, was immediately sent to the Central Research Institute of Nippon Compound Feed Co., Ltd. (currently Feed One Co., Ltd.) and stored at -35°C.

② Storage Location：

The Central Research Institute of Nippon Compound Feed Co., Ltd. (currently Feed One Co., Ltd.) laboratory (temperature and humidity controlled 24 hours a day): The laboratory environment was controlled to be similar to that of the mouse and rat breeding room (temperature = 20-25°C, humidity = 40-60%). Temperature and humidity during the experiment were recorded using an automatic temperature and humidity recorder.

③ Storage Method：

The diet was placed in the feeding section of a mouse cage lid (mouse PC lid, CL-0103-1PC) without any animals and placed on a rack in the laboratory. Samples were taken immediately after opening (0 days), and then after 1, 2, 4, 7, and 14 days of storage. The sampled feed was sealed in a nylon polyethylene bag and stored at -35°C. Analyses were conducted in batches when a sufficient number of samples had been collected.

④ Analysis Items： AV、POV

Analyses were conducted at the Central Research Institute of Nippon Compound Feed Co., Ltd. (currently Feed One Co., Ltd.). The analysis items were acid value (AV) and peroxide value (POV).

（3）Results

The results are shown in Figures 1 and 2 and Table 3.

Quick Fat: Both AV and POV of Quick Fat increased as the storage period increased. In particular, the increase in POV was remarkable after 4 days of storage.

（4）Discussion

It is considered that the fat in Quick Fat does not change significantly for about 4 days after the feed is placed in the feeder, so there is a high possibility that there will be no adverse effects such as a decrease in palatability. In this test, the test was conducted assuming that the feed was placed in the animal feeder. Please refer to this data when setting the timing (frequency of replacement) for replacing feed when using Quick Fat.

Figures 1 and 2: Changes in Quick Fat after opening

Feed that was opened and left standing in a laboratory with controlled temperature and humidity was sampled at regular intervals, and AV and POV were analyzed to observe changes in the fat in the feed.

Table 3: Changes in Quick Fat after opening

Values in parentheses indicate relative values when 0 days (start) is taken as 100%.

 

3. Changes in Quick Fat in a Paper Bag after Opening (3 months)

（1）Objective

The purpose of this study was to observe changes in the fat in Quick Fat under the condition where the opened bag was stored in the breeding room with the mouth of the bag closed with a rubber band.

（2）Materials and Methods

Feed that was opened and left standing in a laboratory with controlled temperature and humidity was sampled at regular intervals, and the acid value (AV) and peroxide value (POV) were analyzed to observe changes in the fat in the feed.

① Test Feed：

Quick Fat: Produced on June 14, 2004, and immediately sent to the Central Research Institute of Nippon Compound Feed Co., Ltd. (currently Feed One Co., Ltd.) and stored at -35°C.

② Storage Location：

Mouse breeding room of Nippon Compound Feed Co., Ltd. (currently Feed One Co., Ltd.) Central Research Institute (Temperature and humidity controlled 24 hours: Temperature = 20-25°C, Humidity = 40-60%). The temperature and humidity during the test were recorded using an automatic temperature and humidity recorder.

③ Storage Method：

The feed bag was opened and the mouth was tightly closed with a rubber band and left standing. Considering the usage of the feed by the user, it was expected that the feed bag would be opened and closed 1-2 times a week during feeding work. Therefore, the bag was opened and closed once a week during the storage period of this test. Sampling was performed as the opening and closing work on the week of sampling.

④ Storage Period and Sampling Time

The storage period was 3 months, and sampling was performed immediately after opening, and after 7, 14, 30, 60, and 90 days of storage. The sampled feed was sealed in a nylon polyethylene bag (Hiryu) and stored at -35°C, and the samples were collected and analyzed together.

⑤ Analysis Items

AV and POV were each measured at two points, and the average value was used as the value.

（3）Results

The results of the analysis are shown in Figures 3 and 4 and Table 4. The AV value increased as the storage period increased, but no significant change was observed in the POV value. In a separate storage test assuming that the feed was placed in an animal feeder (results shown in Table 3), the feed was left in contact with fresh air for 2 weeks, and the change in fat in Quick Fat was more significant for POV than AV. From the above, it is considered that even if Quick Fat is opened, it can be used for 3 months without any problems even if it is left in the temperature and humidity environment of the breeding room as long as the opening of the bag is closed with a rubber band.

In this test, the test was conducted assuming that the opened bag was stored in the breeding room with the mouth of the bag closed with a rubber band. Please refer to this data when considering the expiration date of opened feed when using Quick Fat.

Figures 3 and 4: Changes in Quick Fat in the feed bag after opening

Feed in a bag with the opening closed with a rubber band was left standing in a laboratory with controlled temperature and humidity, and the feed was sampled at regular intervals to analyze AV and POV and observe changes in the fat in the feed.

Table 4: Changes in Quick Fat in the feed bag after opening

Values in parentheses indicate relative values when 0 days (start) is taken as 100%.