Environmental Control
Animals/About Production and Supply System/
Environmental Control
Environmental control plays an important role in the formation of the Barrier System, in association with microbiologic control. For the purpose of this control, managerial standards are developed not only for the rearing environmental conditions, but also for hygiene control and environment conservation around the Barrier.
| Rearing environmental conditions for mice and rats (Barrier System) | |
|---|---|
| Temperature | 20~26℃ |
| Humidity | Mice, 40~60% Rats, 50~70% |
| Air filter | HEPA filter |
| Ventilation frequency | 10~15 air changes/h |
| Wind velocity | 15~20cm/s |
| Barometric pressure | Positive pressure 5~8mmH20 on the outside |
| Illumination intensity | 200lx/FL+800mm |
| Lighting hours | On: 12h / Off：12h |
| Rearing environmental conditions for monkeys | ||
|---|---|---|
| Temperature | Common marmoset Cynomolgus monkey | 26~30℃ 26~30℃ |
| Humidity | Common marmoset Cynomolgus monkey | 40~60% 45~65% |
| Air filter | Medium performance filter | |
| Ventilation frequency | 10~15 air changes/h | |
| Barometric pressure | Common marmoset Cynomolgus monkey | Positive pressure of 100-200 Pa on the outside Negative pressure of 200-400 Pa on the outside |
| Lighting hours | On: 12h / Off：12h | |
| Methods of disinfection and sterilization of humans and major materials in Barrier System | |
|---|---|
| Humans | Warm water shower (use of antiseptic solution for hands and feet) |
| Diet | Autoclaved sterilization (121℃, 30 min), Radiantion sterilization (30kGy) |
| Water | Adding 4~6 ppm chlorine, boiling (partly) |
| Cages | Autoclaved sterilization (121℃, 30 min), Immersion in 100ppm chlorine |
| Bedding materials | Autoclaved sterilization (121℃, 30 min) |
| Transport box | EOG sterilization (4 h or longer exposure) |
| Inside of the facility | Spraying of drung solutin (chlorine, iodophor, invert soap, ethanol, peracetic acid solution, etc.), formalin gas fumiagation |