Kras301/Jcl
Animals/Genetically Modified Animals/
Ordering name: Kras301/Jcl
Nomenclature: SD-Tg(KRASflox/flox)301/Jcl
Availability: Cryopreserved
Genetically modified animal (subject to the Cartagena Protocol on Biosafety)
(*This strain is kept limited numbers and supplied only from its surplus. If you want more than that, please request us to expand.)
Origin
Kras301 rat is a conditional transgenic rat introduced mutated form human origin K-ras gene to Jcl:SD rat embryo by Dr. Hiroyuki Tsuda, Nagoya University in 2005.
This model had been maintained and developed at CLEA Japan facility as a breeding service for certain research organizations. Then we started selling it as a commercial sales model according to the agreement between Dr. Tsuda.
Characteristics
This strain is introduced 3 copies of hemagglutinin (HA) epitope tag connected HA-K-rasv12 gene expression cassette by using Cre/loxP system for controlling term/location expression of human mutated form K-rasv12 gene (changing glycine to valine at the 12 amino acid sequence) that normally floxed rat does not express K-rasv12 gene.
It can be created an organ-specific cancer expression in short term by the human mutated form K-rasv12 gene expression by erasing a neomycin-resistant gene caught in the loxP by infecting an adenoviral vector, express Cre recombinase* at the subject organs. Especially it can be expressed pancreatic carcinoma, one of the intractable cancer and difficult to detect early, by express Cre recombinase at pancreatic duct.
(*P2 level)
This strain is kept as a homo type (tg/tg), male Tg rat and female Tg rat mating.
◆References(*Japanese)
Our Contract Research Services related with This Animal
For details on our contract research services, including cryopreserved embryos, contracted testing, and the provision of research materials such as blood and organs, please click here .