Diabetes Model Information-1
Animals/Diabetes Models/
Diabetes model information -1
Blood glucose levels of type 2 diabetes model
Diet: CE-2 (autoclaved sterilization at 121°C, 30 min), freely available
Method of rearing: Isolation rearing for KK, KK-Ay
Site of blood collection: mouse, orbital venous plexus; rat, caudal vein
Samples: mouse, plasma; rat, whole blood
Points to consider when rearing and conducting experiments using diabetes model animals
- Animals should first be reared for 3 - 5 days to provide sufficient environmental acclimation prior to the start of experiments because the animals may exhibit decreased blood glucose levels as a result of stress experienced during transport, etc.
- Isolated rearing is recommended for KK-Ay mice because these mice may not exhibit an increase in blood glucose levels because of fighting, etc., if they are reared in a group.
- Because animals become polyuric after developing diabetes, frequent changes and large amounts of bedding materials are recommended.
- Because the blood glucose level varies according to diet, measurement method, and type of blood sample (blood, serum, plasma), measurements should be performed using a standard method.